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STEMCELL Technologies Inc bit 9500 serum substitute
Bit 9500 Serum Substitute, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bit+9500+serum+substitute/bit+9500/bio_rxiv__2025__07__15__664865-166-25-33
Average 90 stars, based on 1 article reviews
bit 9500 serum substitute - by Bioz Stars, 2026-09
90/100 stars

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Cell Culture:

Article Title: Mitochondrial DNA Mutations Determine Favourable Molecular Responses to Targeted Kinase Inhibitor Therapy and Impair Oxidative Phosphorylation
Article Snippet: .. Primary samples were cultured at 37°C and 5% CO2 in serum-free media (SFM) consisting of Iscove’s modified Dulbecco medium (Sigma-Aldrich, Missouri, United Stated) supplemented with BIT 9500 Serum Substitute (bovine serum albumin, BSA/insulin/transferrin; STEMCELL Technologies, Vancouver, Canada), 2 mM L-Glutamine (Sigma-Aldrich, St. Louis, Missouri, United States), 1 mM streptomycin/penicillin, 0.1 mM 2-mercaptoethanol, 0.02 mg/mL human Low Density Lipoprotein (STEMCELL Technologies, Vancouver, Canada). .. The SFM was then filtered and supplemented with a physiological growth factor cocktail (STEMCELL Technologies, Vancouver, Canada) consisting of 0.05 ng/mL human recombinant LIF, 1 ng/mL G-CSF and 0.2 ng/mL SCF/GM-CSF/MIP-α/IL-6.

Article Title: Disassembly of self-assembling peptide hydrogels as a versatile method for cell extraction and manipulation
Article Snippet: .. Hematopoietic stem cells (HSCs) were isolated from human bone marrow and cultured in Iscove's modified Dulbecco medium (IMDM) containing 20% BIT 9500 serum substitute (STEMCELL Technologies), 1% 200 mM l -glutamine (Sigma-Aldrich), 1% 10 mg mL −1 penicillin/streptomycin (Invitrogen) supplemented with Flt3L (50 ng mL −1 ), SCF (50 ng mL −1 ) and TPO (25 ng mL −1 ). ..

Article Title: Transcriptional and epigenetic rewiring by the NUP98::KDM5A fusion oncoprotein directly activates CDK12
Article Snippet: Platinum-E (Cell Biolabs, USA) and Lenti-X 293 T cells (Takara, France) were cultivated in DMEM (Gibco, USA) supplemented with 10% FBS, 100 U/ml penicillin, 100 μg/ml streptomycin and 2 or 4 mM l -Glutamine, respectively. .. Primary patient-derived leukemia cells, human BM CD34+ Progenitor Cells (#2M-101C) and Human BM MNC (#2M-125C) (Lonza, Switzerland) and PDX cells were cultured in IMDM (Gibco, USA) containing 15% BIT 9500 Serum Substitute (STEMCELL Technologies, Canada), 100 ng/ml SCF, 50 ng/ml Flt3-Ligand, 20 ng/ml IL-3, 20 ng/ml G-CSF (all from PeproTech, Austria), 100 μM 2-Mercaptoethanol, 50 μg/ml gentamicin (Gibco, USA), and 10 μg/ml ciprofloxacin (Merck, Germany) plus 500 nM SR1 (APExBIO, USA) and 1 μm UM729 (APExBIO, USA) as previously described . ..

Article Title: Pro-inflammatory macrophages suppress HIV replication in humanized mice and ex vivo co-cultures.
Article Snippet: .. For the transduction, CD34+ cells were cultured in Isove’s modified eagle’s medium (Thermo Fisher Scientific, Cat#: 12440053) supplemented with 20% BIT 9500 serum substitute (Stemcell technologies, Cat#: 09500), 10 mg/ml human low-density lipoprotein (Stemcell technologies, Cat#: 02698), 0.1mM 2-mercaptoethanol (Sigma Aldrich, Cat#: 63689-25ML-F), 0.1mM Glutamax 500 (Glibco, Cat#: 35050087), 100 ng/ml FLT3-L, 100 ng/ml SCF, 20 ng/ml IL-3, 20 ng/ml IL-6, 20 ng/ml G-CSF (all Immunotools, Cat#: 11343303, 11343325, 11340033, 11340064, 11343133) and UM171 35nM. .. CD34+ cells were transduced 12 hrs after isolation at a MOI of 5, in the presence of LentiBlast Premium (1:500) (OZ Biosciences, Cat#: LBPX500) and medium was changed 16 hrs later.

Article Title: Bioengineered niches that recreate physiological extracellular matrix organisation to support long-term haematopoietic stem cells
Article Snippet: CD34 +ve cells from human BM were purchased from CalTag Medystems (experiments related to Fig. ) and STEMCELL Technologies or isolated from BM samples as above (Fig. ). .. CD34 +ve cells were cultured in serum-free medium; Iscove’s Modified Dulbecco Medium (IMDM) containing 20% BIT 9500 serum substitute (STEMCELL Technologies), 1% 200 mM L-glutamine (Sigma-Aldrich), 1% 10 mg/ml penicillin/streptomycin (Invitrogen) supplemented with Flt3L (50 ng/ml), SCF (20 ng/ml) and TPO (25 ng/ml) (all recombinant human, Peprotech, 300-19, 300-07, 300-18) (100% medium). ..

Article Title: Sphingosine-1 phosphate receptor 1 (S1PR1) expression maintains stemness of acute myeloid leukemia stem cells.
Article Snippet: .. 4 mL of bone marrow blood from patients with AML at diagnosis and healthy donors were cultured in IMDM (ThermoFisher Scientific, USA) supplemented with 20 % BIT 9500 Serum Substitute (Stem Cell Technologies, Canada). .. Bone marrow mononuclear cells were stained with CD34-PE (Cat# 550761, BD Biosciences, USA) and S1PR1-APC (Cat# 50-3639-42, ThermoFisher Scientific, USA) for 15 min at room temperature.

Article Title: Alisertib inhibits acute myeloid leukemia cell growth by inhibiting STAT3 activation.
Article Snippet: Aurora kinase A (AURKA) plays critical roles in the cell cycle.. Its oncogenic functions have been identified in various types of cancer.. However, its role in acute myeloid leukemia (AML) has not been extensively explored.

Modification:

Article Title: Mitochondrial DNA Mutations Determine Favourable Molecular Responses to Targeted Kinase Inhibitor Therapy and Impair Oxidative Phosphorylation
Article Snippet: .. Primary samples were cultured at 37°C and 5% CO2 in serum-free media (SFM) consisting of Iscove’s modified Dulbecco medium (Sigma-Aldrich, Missouri, United Stated) supplemented with BIT 9500 Serum Substitute (bovine serum albumin, BSA/insulin/transferrin; STEMCELL Technologies, Vancouver, Canada), 2 mM L-Glutamine (Sigma-Aldrich, St. Louis, Missouri, United States), 1 mM streptomycin/penicillin, 0.1 mM 2-mercaptoethanol, 0.02 mg/mL human Low Density Lipoprotein (STEMCELL Technologies, Vancouver, Canada). .. The SFM was then filtered and supplemented with a physiological growth factor cocktail (STEMCELL Technologies, Vancouver, Canada) consisting of 0.05 ng/mL human recombinant LIF, 1 ng/mL G-CSF and 0.2 ng/mL SCF/GM-CSF/MIP-α/IL-6.

Article Title: Disassembly of self-assembling peptide hydrogels as a versatile method for cell extraction and manipulation
Article Snippet: .. Hematopoietic stem cells (HSCs) were isolated from human bone marrow and cultured in Iscove's modified Dulbecco medium (IMDM) containing 20% BIT 9500 serum substitute (STEMCELL Technologies), 1% 200 mM l -glutamine (Sigma-Aldrich), 1% 10 mg mL −1 penicillin/streptomycin (Invitrogen) supplemented with Flt3L (50 ng mL −1 ), SCF (50 ng mL −1 ) and TPO (25 ng mL −1 ). ..

Article Title: Pro-inflammatory macrophages suppress HIV replication in humanized mice and ex vivo co-cultures.
Article Snippet: .. For the transduction, CD34+ cells were cultured in Isove’s modified eagle’s medium (Thermo Fisher Scientific, Cat#: 12440053) supplemented with 20% BIT 9500 serum substitute (Stemcell technologies, Cat#: 09500), 10 mg/ml human low-density lipoprotein (Stemcell technologies, Cat#: 02698), 0.1mM 2-mercaptoethanol (Sigma Aldrich, Cat#: 63689-25ML-F), 0.1mM Glutamax 500 (Glibco, Cat#: 35050087), 100 ng/ml FLT3-L, 100 ng/ml SCF, 20 ng/ml IL-3, 20 ng/ml IL-6, 20 ng/ml G-CSF (all Immunotools, Cat#: 11343303, 11343325, 11340033, 11340064, 11343133) and UM171 35nM. .. CD34+ cells were transduced 12 hrs after isolation at a MOI of 5, in the presence of LentiBlast Premium (1:500) (OZ Biosciences, Cat#: LBPX500) and medium was changed 16 hrs later.

Article Title: Bioengineered niches that recreate physiological extracellular matrix organisation to support long-term haematopoietic stem cells
Article Snippet: CD34 +ve cells from human BM were purchased from CalTag Medystems (experiments related to Fig. ) and STEMCELL Technologies or isolated from BM samples as above (Fig. ). .. CD34 +ve cells were cultured in serum-free medium; Iscove’s Modified Dulbecco Medium (IMDM) containing 20% BIT 9500 serum substitute (STEMCELL Technologies), 1% 200 mM L-glutamine (Sigma-Aldrich), 1% 10 mg/ml penicillin/streptomycin (Invitrogen) supplemented with Flt3L (50 ng/ml), SCF (20 ng/ml) and TPO (25 ng/ml) (all recombinant human, Peprotech, 300-19, 300-07, 300-18) (100% medium). ..

Isolation:

Article Title: Disassembly of self-assembling peptide hydrogels as a versatile method for cell extraction and manipulation
Article Snippet: .. Hematopoietic stem cells (HSCs) were isolated from human bone marrow and cultured in Iscove's modified Dulbecco medium (IMDM) containing 20% BIT 9500 serum substitute (STEMCELL Technologies), 1% 200 mM l -glutamine (Sigma-Aldrich), 1% 10 mg mL −1 penicillin/streptomycin (Invitrogen) supplemented with Flt3L (50 ng mL −1 ), SCF (50 ng mL −1 ) and TPO (25 ng mL −1 ). ..

Purification:

Article Title: Developmental interplay between transcriptional alterations and a targetable cytokine signaling dependency in pediatric ETO2::GLIS2 leukemia
Article Snippet: .. Purified CD34 + cells (or hematopoietic subfractions, 1 × 10 6 cells/mL) were seeded in BIT 9500 Serum Substitute (StemCell Technologies) supplemented with 1% P/S, 100 ng/mL hSCF, 100 ng/mL hFLT3L, 60 ng/mL hIL3 and 200 ng/mL hTPO (all from Miltenyi Biotec) [ ]. ..

Transduction:

Article Title: Pro-inflammatory macrophages suppress HIV replication in humanized mice and ex vivo co-cultures.
Article Snippet: .. For the transduction, CD34+ cells were cultured in Isove’s modified eagle’s medium (Thermo Fisher Scientific, Cat#: 12440053) supplemented with 20% BIT 9500 serum substitute (Stemcell technologies, Cat#: 09500), 10 mg/ml human low-density lipoprotein (Stemcell technologies, Cat#: 02698), 0.1mM 2-mercaptoethanol (Sigma Aldrich, Cat#: 63689-25ML-F), 0.1mM Glutamax 500 (Glibco, Cat#: 35050087), 100 ng/ml FLT3-L, 100 ng/ml SCF, 20 ng/ml IL-3, 20 ng/ml IL-6, 20 ng/ml G-CSF (all Immunotools, Cat#: 11343303, 11343325, 11340033, 11340064, 11343133) and UM171 35nM. .. CD34+ cells were transduced 12 hrs after isolation at a MOI of 5, in the presence of LentiBlast Premium (1:500) (OZ Biosciences, Cat#: LBPX500) and medium was changed 16 hrs later.

Recombinant:

Article Title: Bioengineered niches that recreate physiological extracellular matrix organisation to support long-term haematopoietic stem cells
Article Snippet: CD34 +ve cells from human BM were purchased from CalTag Medystems (experiments related to Fig. ) and STEMCELL Technologies or isolated from BM samples as above (Fig. ). .. CD34 +ve cells were cultured in serum-free medium; Iscove’s Modified Dulbecco Medium (IMDM) containing 20% BIT 9500 serum substitute (STEMCELL Technologies), 1% 200 mM L-glutamine (Sigma-Aldrich), 1% 10 mg/ml penicillin/streptomycin (Invitrogen) supplemented with Flt3L (50 ng/ml), SCF (20 ng/ml) and TPO (25 ng/ml) (all recombinant human, Peprotech, 300-19, 300-07, 300-18) (100% medium). ..

Biomarker Discovery:

Article Title: Sphingosine-1 phosphate receptor 1 (S1PR1) expression maintains stemness of acute myeloid leukemia stem cells.
Article Snippet: .. 4 mL of bone marrow blood from patients with AML at diagnosis and healthy donors were cultured in IMDM (ThermoFisher Scientific, USA) supplemented with 20 % BIT 9500 Serum Substitute (Stem Cell Technologies, Canada). .. Bone marrow mononuclear cells were stained with CD34-PE (Cat# 550761, BD Biosciences, USA) and S1PR1-APC (Cat# 50-3639-42, ThermoFisher Scientific, USA) for 15 min at room temperature.



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